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SsoAdvanced? universal SYBR? Green supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.
SsoAdvanced? universal SYBR? Green supermix lets you:
- Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
- Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
- Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
- Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
- Obtain better results with Bio-Rad's PrimePCR? assays — real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance
Applications and Uses of SsoAdvanced? Universal SYBR? Green Supermix
- qPCR/real-time PCR
- Gene expression analysis
- Pathway analysis
- Absolute quantification
- Chromatin immunoprecipitation (ChIP) qPCR
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Characterization of genetically modified organisms (GMO)
- Genetic profiling
Packaging Options
| Number of 20 μl Reactions | Total Volume | Number of Vials | Catalog # |
| 200 | 2 ml | 2 x 1 ml | #172-5270 |
| 500 | 5 ml | 5 x 1 ml | #172-5271 |
| 1,000 | 10 ml | 10 x 1 ml | #172-5272 |
| 2,500 | 25 ml | 5 x 5 ml | #172-5274 |
| 5,000 | 50 ml | 10 x 5 ml | #172-5275 |
Instrument Compatibility
The SsoAdvanced? universal SYBR? Green supermix is compatible with all commercially available and allBio-Rad real-time qPCR systems.
Related Products
We have developed SsoAdvanced universal probes supermix, which is available in several formats (200–5,000 reactions).
Bio-Rad offers a complete workflow solution for real-time PCR, including:
More Information
To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.